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af5779 rrid ab 1964666  (R&D Systems)


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    R&D Systems af5779 rrid ab 1964666
    Af5779 Rrid Ab 1964666, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/af5779+rrid+ab+1964666/Human+p16INK4a%2F+CDKN2A+Antibody/pmc13092703-76-86-83
    Average 93 stars, based on 20 article reviews
    af5779 rrid ab 1964666 - by Bioz Stars, 2026-09
    93/100 stars

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    Article Title: A Three-subtype Molecular model of Cervical Cancer: Multiple PI3K Pathway inhibitors suppress growth and cooperate with HPV-directed immunotherapy
    Article Snippet: In total, 20 - 40 micrograms of whole cell protein were separated in a 4–12% NuPAGE Bis-Tris-gel, transferred to PVDF membrane (Thermo Fisher Scientific, Carlsbad, CA, United States), and immunoblotted with primary antibodies in 0.05% Tween 20-Tris-buffered saline (TBST) containing 5% skim milk at 4°C with shaking overnight. .. The primary antibodies used in this study were: rabbit monoclonal anti-PD-L1, (Cell Signaling Technology Cat# 13684, RRID: AB_2687655), YAP1, (Cell Signaling Technology Cat# 14074, RRID: AB_2650491), EGFR (Cell Signaling Technology Cat# 4267, RRID: AB_2800085), CTGF (Cell Signaling Technology Cat# 86641, RRID: AB_2800085), Integrin α6 (Cell Signaling Technology Cat# 3750, RRID: AB_2249263), IRF1 (Cell Signaling Technology Cat# 8478, RRID: AB_10949108) at a dilution of 1:500; mouse monoclonal anti-HPV16 E7 at 1:100 (Santa Cruz Cat# sc-51951, RRID: AB_629662), and goat monoclonal anti-p16 at 1:500 (R&D system Cat# AF5779, RRID: AB_1964666). β-actin, used as an internal control, was detected by rabbit anti-β-actin antibody (Cell Signaling Technology Cat# 4967, RRID: AB_330288). .. Anti-rabbit IgG, HRP-linked Antibody (Cell Signaling Technology Cat# 7074, RRID: AB_2099233) and Anti-mouse IgG, HRP-linked Antibody at 1:3000 - 1:5000 (Cell Signaling Technology Cat# 7076, RRID: AB_330924) were used as a secondary antibody.

    Article Title: PIK3CA mutant cervical cancer is selectively suppressed by PI3Kα inhibition (Alpelisib/BYL-719 and Inavolisib/GDC-0077) and cooperates with HPV directed T cell therapy.
    Article Snippet: In total, 20 - 40 micrograms of whole cell protein were separated in a 4–12% NuPAGE Bis-Tris-gel, transferred to PVDF membrane (Thermo Fisher Scientific, Carlsbad, CA, United States), and immunoblotted with primary antibodies in 0.05% Tween 20-Tris-buffered saline (TBST) containing 5% BSA at 4◦C with shaking overnight. .. The primary antibodies used in this study were: rabbit monoclonal anti-PD-L1, (Cell Signaling Technology Cat# 13684, RRID: AB_2687655), YAP1, (Cell Signaling Technology Cat# 14074, RRID: AB_2650491), EGFR (Cell Signaling Technology Cat# 4267, RRID: AB_2800085), CTGF (Cell Signaling Technology Cat# 86641, RRID: AB_2800085), Integrin α6 (Cell Signaling Technology Cat# 3750, RRID: AB_2249263), IRF1 (Cell Signaling Technology Cat# 8478, RRID: AB_10949108) at a dilution from1:250 to 1:500; mouse monoclonal anti-HPV16 E7 at 1:100 (Santa Cruz Cat# sc-51951, RRID: AB_629662), and goat monoclonal anti-p16 at 1:500 (R&D system Cat# AF5779, RRID: AB_1964666). β-actin, used as an internal control, was detected by rabbit anti-β-actin antibody (Cell Signaling Technology Cat# 4967, RRID: AB_330288). .. Anti-rabbit IgG, HRP-linked Antibody (Cell Signaling Technology Cat# 7074, RRID: AB_2099233) and Anti-mouse IgG, HRP-linked Antibody at 1:3000 - 1:5000 (Cell Signaling Technology Cat# 7076, RRID: AB_330924) were used as a secondary antibody.

    Article Title: PIK3CA mutant cervical cancer is selectively suppressed by PI3Kα inhibition (Alpelisib/BYL-719 and Inavolisib/GDC-0077) and cooperates with HPV directed T cell therapy
    Article Snippet: In total, 20 - 40 micrograms of whole cell protein were separated in a 4–12% NuPAGE Bis-Tris-gel, transferred to PVDF membrane (Thermo Fisher Scientific, Carlsbad, CA, United States), and immunoblotted with primary antibodies in 0.05% Tween 20-Tris-buffered saline (TBST) containing 5% BSA at 4°C with shaking overnight. .. The primary antibodies used in this study were: rabbit monoclonal anti-PD-L1, (Cell Signaling Technology Cat# 13684, RRID: AB_2687655), YAP1, (Cell Signaling Technology Cat# 14074, RRID: AB_2650491), EGFR (Cell Signaling Technology Cat# 4267, RRID: AB_2800085), CTGF (Cell Signaling Technology Cat# 86641, RRID: AB_2800085), Integrin α6 (Cell Signaling Technology Cat# 3750, RRID: AB_2249263), IRF1 (Cell Signaling Technology Cat# 8478, RRID: AB_10949108) at a dilution from1:250 to 1:500; mouse monoclonal anti-HPV16 E7 at 1:100 (Santa Cruz Cat# sc-51951, RRID: AB_629662), and goat monoclonal anti-p16 at 1:500 (R&D system Cat# AF5779, RRID: AB_1964666). β-actin, used as an internal control, was detected by rabbit anti-β-actin antibody (Cell Signaling Technology Cat# 4967, RRID: AB_330288). .. Anti-rabbit IgG, HRP-linked Antibody (Cell Signaling Technology Cat# 7074, RRID: AB_2099233) and Anti-mouse IgG, HRP-linked Antibody at 1:3000 - 1:5000 (Cell Signaling Technology Cat# 7076, RRID: AB_330924) were used as a secondary antibody.



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    af5779 rrid ab 1964666  (R&D Systems)
    93
    R&D Systems af5779 rrid ab 1964666
    Af5779 Rrid Ab 1964666, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/af5779+rrid+ab+1964666/Human+p16INK4a%2F+CDKN2A+Antibody/pmc13092703-76-86-83
    Average 93 stars, based on 1 article reviews
    af5779 rrid ab 1964666 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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